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Journal / Biochemistry / Split-BioID — Proteom analyse af…
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Biochemistry

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JoVE Journal Biochemistry
Split-BioID — Proteom analyse af kontekst-specifik proteinkomplekser i deres Native cellulære miljø
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Split-BioID — Proteom analyse af kontekst-specifik proteinkomplekser i deres Native cellulære miljø

Article DOI: 10.3791/57479-v • 09:02 min • April 20th, 2018
April 20th, 2018 •
1,2, 1,2
1Cluster of excellence CellNetworks, Heidelberg University, 2Heidelberg University Biochemistry Center (BZH)

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Vi leverer en trinvis protokol for split-BioID, en protein fragmenter-komplementering analyse baseret på den nærhed-mærkning teknik BioID. Aktiveret på samspillet mellem to givne proteiner, tillader det proteomics analysen af kontekst-afhængige proteinkomplekser i deres native cellulære miljø. Metoden er enkel, omkostningseffektiv og kræver kun almindeligt laboratorieudstyr.

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Biokemi spørgsmål 134 BioID proteomics protein engineering protein-fragmenter komplementering analyse protein-protein interaktioner nærhed mærkning
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