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Journal / Biochemistry / Split-BioID-Proteomic analyse av…
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Biochemistry

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JoVE Journal Biochemistry
Split-BioID-Proteomic analyse av kontekstavhengige Protein komplekser i sitt opprinnelige mobilnettet miljø
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Split-BioID-Proteomic analyse av kontekstavhengige Protein komplekser i sitt opprinnelige mobilnettet miljø

Article DOI: 10.3791/57479-v • 09:02 min • April 20th, 2018
April 20th, 2018 •
1,2, 1,2
1Cluster of excellence CellNetworks, Heidelberg University, 2Heidelberg University Biochemistry Center (BZH)

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Vi gir en trinnvis protokoll for split-BioID, en protein fragmenter-complementation analysen basert på nærhet-merking teknikken BioID. Aktivert på samspillet av to gitt proteiner, kan Proteomikk analyse av sammenhengen-avhengige proteiner komplekser i sitt opprinnelige mobilnettet miljø. Metoden er enkel, kostnadseffektiv og krever bare standard laboratorieutstyr.

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Biokjemi problemet 134 BioID Proteomikk protein engineering protein-fragmenter complementation analysen protein-protein interaksjoner nærhet merking
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