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Generation av mänsklig Nasal epitelial Cell Spheroids för individualiserad cystisk fibros Transmembrane Conductance Regulator studie
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Generation av mänsklig Nasal epitelial Cell Spheroids för individualiserad cystisk fibros Transmembrane Conductance Regulator studie

Article DOI: 10.3791/57492-v • 08:00 min • April 11th, 2018
April 11th, 2018 •
1, 1, 1, 1, 1, 1, 1
1Department of Pediatrics, Division of Pulmonary Medicine, Cincinnati Children's Hospital Medical Center

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Här beskriver vi en metod för att skapa tredimensionella sfäroid kulturer av mänskliga näsans epitelceller. Spheroids stimuleras då att köra cystisk fibros Transmembrane Conductance Regulator (CFTR)-beroende ion och vätska sekretion, kvantifiera förändringen i sfäroid luminala storleken som en proxy för CFTR funktion.

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Medicin fråga 134 cystisk fibros CFTR Precision Medicine Organoid sfäroid Nasal Cell cellkultur
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