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Generering av menneskelig nese tarmepitelet Spheroids for individualisert cystisk fibrose Transmembrane konduktans Regulator studie
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Generering av menneskelig nese tarmepitelet Spheroids for individualisert cystisk fibrose Transmembrane konduktans Regulator studie

Article DOI: 10.3791/57492-v • 08:00 min • April 11th, 2018
April 11th, 2018 •
1, 1, 1, 1, 1, 1, 1
1Department of Pediatrics, Division of Pulmonary Medicine, Cincinnati Children's Hospital Medical Center

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Her beskriver vi en metode for å generere tredimensjonalt formet kulturer av menneskelig nese epitelceller. Spheroids deretter stimulert til stasjonen cystisk fibrose Transmembrane konduktans Regulator (CFTR)-avhengige ion og væske sekresjon, kvantifisere endringen i spheroid luminal størrelsen som en proxy for CFTR funksjon.

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Medisin problemet 134 cystisk fibrose CFTR Precision medisin Organoid Spheroid Nasal celle cellekultur
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