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JoVE Journal Bioengineering
Microiniezione di Western Corn Rootworm, Diabrotica virgifera virgifera, embrioni per la trasformazione di Germline o CRISPR/Cas9 Editing genomico
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Microiniezione di Western Corn Rootworm, Diabrotica virgifera virgifera, embrioni per la trasformazione di Germline o CRISPR/Cas9 Editing genomico

Article DOI: 10.3791/57497-v • 07:42 min • April 27th, 2018
April 27th, 2018 •
1, 1, 1, 1, 1
1Department of Entomology and Plant Pathology, North Carolina State University

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Qui presentiamo protocolli per la raccolta e microinjecting mais precellular embrioni di Diabrotica allo scopo di eseguire dosaggi funzionali-genomica come trasformazione di germline ed editing CRISPR/Cas9-genomico.

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Bioingegneria problema 134 microinjection embrionale genomica funzionale trasformazione di germline transgenesi CRISPR/Cas9 editing genomico diabrotica del mais
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