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JoVE Journal Genetics
Gene målrettede tilfeldig mutagenese Velg Heterochromatin-destabilisere Proteasome mutanter fisjon gjær
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Gene målrettede tilfeldig mutagenese Velg Heterochromatin-destabilisere Proteasome mutanter fisjon gjær

Article DOI: 10.3791/57499-v • 07:18 min • May 15th, 2018
May 15th, 2018 •
1, 1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

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Denne artikkelen beskriver en detaljert metodikk for tilfeldige mutagenese med et mål i fisjon gjær. Som et eksempel målet vi rpt4 +, som koder en undergruppe av 19S proteasome og skjermen for mutasjoner som destabilisere heterochromatin.

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Genetikk problemet 135 tilfeldig mutagenese fisjon gjær heterochromatin utsatt for feil PCR centromere genet målrette
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