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Journal / Neuroscience / Gør, afprøvning og brug af kalium Ion selektive…
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JoVE Journal Neuroscience
Gør, afprøvning og brug af kalium Ion selektive Microelectrodes i væv skiver af voksne hjerne
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Gør, afprøvning og brug af kalium Ion selektive Microelectrodes i væv skiver af voksne hjerne

Article DOI: 10.3791/57511-v • 11:20 min • May 7th, 2018
May 7th, 2018 •
1, 2, 1,2, 1,3
1Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, 2Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, 3Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles

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Kalium ioner bidrage til hvile membranen potentiale celler og ekstracellulære K+ koncentration er en afgørende regulator af cellulære ophidselse. Vi beskriver hvordan man laver, kalibrere og bruge monopolære K+-selektive microelectrodes. Ved hjælp af sådanne elektroder kan måling af elektrisk evoked K+ koncentration dynamics i voksen hippocampus skiver.

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Neurovidenskab spørgsmålet 135 kalium K + ion-selektiv mikroelektrode neurovidenskab Elektrofysiologi hjernen skive astrocyte mus homøostase synapser
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