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JoVE Journal Neuroscience
Maken, testen en het gebruik van kalium-Ion selectieve Microelectrodes in weefsel plakjes van volwassen hersenen
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Maken, testen en het gebruik van kalium-Ion selectieve Microelectrodes in weefsel plakjes van volwassen hersenen

Article DOI: 10.3791/57511-v • 11:20 min • May 7th, 2018
May 7th, 2018 •
1, 2, 1,2, 1,3
1Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, 2Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, 3Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles

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Kalium ionen bijdragen tot de rustpotentiaal membraan van cellen en extracellulaire K+ concentratie is een cruciale regulator van cellulaire prikkelbaarheid. We beschrijven hoe te maken, te kalibreren en te gebruiken monopolaire K+-selectieve microelectrodes. Deze elektroden, kunt de meting van elektrisch evoked K+ concentratie dynamiek in volwassen hippocampal plakjes.

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Neurowetenschappen kwestie 135 kalium K + ion-selectieve micro-elektrode neurowetenschap elektrofysiologie hersenen slice Astrocyt muis homeostase synapsen
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