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Virus de la entrega de guías CRISPR para la próstata murina por alteración del gen
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Virus de la entrega de guías CRISPR para la próstata murina por alteración del gen

Article DOI: 10.3791/57525-v • 06:21 min • April 27th, 2018
April 27th, 2018 •
1, 1, 2, 2, 1
1Department of Clinical Medicine, Aarhus University, 2GDD, Cancer Cell Biology Program, National Cancer Research Center (CNIO)

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Este protocolo describe un método nuevo para la entrega del virus a la próstata murina. Usando tecnología CRISPR/Cas9, sobreexpresión del gen o entrega del recombinase de Cre, la técnica permite orthotopic alteración de la expresión génica e implementa un modelo novedoso ratón para cáncer de próstata.

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Cancer Research número 134 cáncer de próstata la edición del genoma terapia génica alteración de genes entrega de virus inyección de virus virus adeno-asociado CRISPR/Cas9 vivo en tumor modelo iniciación del tumor
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