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Journal / Neuroscience / Identifisere transkripsjon faktor Olig2 genomisk…
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Neuroscience

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JoVE Journal Neuroscience
Identifisere transkripsjon faktor Olig2 genomisk bindende områder i akutt renset PDGFRα + celler av lav-cellers Chromatin Immunoprecipitation sekvensering analyse
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Identifisere transkripsjon faktor Olig2 genomisk bindende områder i akutt renset PDGFRα + celler av lav-cellers Chromatin Immunoprecipitation sekvensering analyse

Article DOI: 10.3791/57547-v • 12:29 min • April 16th, 2018
April 16th, 2018 •
1, 1, 1, 1
1The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center

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Her presenterer vi en protokoll som er utformet for å analysere genomet hele binding av oligodendrocyte transkripsjon faktor 2 (Olig2) i akutt renset hjernen oligodendrocyte precursor celler (OPCs) ved å utføre lav-cellers chromatin immunoprecipitation (ChIP) , bibliotek forberedelse, høy gjennomstrømming sekvensering og bioinformatic dataanalyse.

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Nevrovitenskap problemet 134 OPCs Olig2 lav-celle ChIP-seq transcriptional regulering immunopanning MACS2
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