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Adeno-associerade Virus-medierad leverans av CRISPR för hjärt gen redigering i möss
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Adeno-associerade Virus-medierad leverans av CRISPR för hjärt gen redigering i möss

Article DOI: 10.3791/57560-v • 09:00 min • August 2nd, 2018
August 2nd, 2018 •
1, 1, 1, 1
1Department of Surgery, Davis Heart and Lung Research Institute, Biomedical Sciences Graduate Program, Biophysics Graduate Program, Ohio State University Wexner Medical Center

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Här tillhandahåller vi ett detaljerat protokoll att utföra i vivo redigering hjärt genen i möss med rekombinant Adeno-Associated Virus (rAAV)-medierad leverans av CRISPR. Detta protokoll erbjuder en lovande terapeutisk strategi för att behandla dystrofa kardiomyopati Duchennes muskeldystrofi och kan användas för att generera hjärt-specifika knockout i postnatal möss.

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Medicin fråga 138 AAV CRISPR Duchennes muskeldystrofi dystrofin gen redigering mdx rekombinant adeno-associerade virus
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