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JoVE Journal Biochemistry
Trans-Plasma da membrana, transporte de elétrons por Myotubes de C2C12 de medição
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Trans-Plasma da membrana, transporte de elétrons por Myotubes de C2C12 de medição

Article DOI: 10.3791/57565-v • 10:27 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 1
1Department of Biology, Saint Louis University
* These authors contributed equally

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O objetivo do presente protocolo é espectrofotometricamente monitorar o transporte de elétrons da membrana trans-plasma utilizando aceitadores de electrões extracelular e analisar interações enzimáticas que podem ocorrer com estes aceitadores de electrões extracelular.

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Bioquímica edição 135 tPMET WST-1 DPIP ascorbato superóxido C2C12 myotubes ensaio espectrofotométrico
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