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Journal / Biology / 从小鼠眼中激光捕捉高纯大梁网的基因表达分析…
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从小鼠眼中激光捕捉高纯大梁网的基因表达分析
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从小鼠眼中激光捕捉高纯大梁网的基因表达分析

Article DOI: 10.3791/57576-v • 13:47 min • June 3rd, 2018
June 3rd, 2018 •
*1, *2, *1, 2, 2, 2,3, 2,4, 1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH, 2Cellular and Molecular Pathology Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, NIH, 3Integrated Laboratory Systems Inc., 4Experimental Pathology Laboratories Inc.
* These authors contributed equally

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在这里, 我们描述了一个可重现的激光捕获显微切割 (LCM) 的协议, 用于分离小梁网 (TM) 的下游 RNA 分析。分析 tm 基因表达变化的能力有助于了解 tm 相关眼部疾病的基本分子机制。

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生物学 问题 136 青光眼 小梁网 激光捕获显微切割 激光显微切割 RNA 分离 基因表达 LCM LMD
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