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Molekulare Analyse der endothelialen Mesenchymale Transition induziert durch Transforming Growth Factor-β-Signalisierung
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Molekulare Analyse der endothelialen Mesenchymale Transition induziert durch Transforming Growth Factor-β-Signalisierung

Article DOI: 10.3791/57577-v • 07:49 min • August 3rd, 2018
August 3rd, 2018 •
1, 2,3, 4, 2
1David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 2Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, 3Hastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, 4Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo

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Ein Protokoll für in-vitro- Induktion von endothelial-Mesenchymale Transition (EndMT), die nützlich für die Untersuchung von zelluläre Signalwege an EndMT beteiligt ist, wird beschrieben. In diesem experimentellen Modell ist EndMT durch Behandlung mit TGF-β in MS-1 Endothelzellen induziert.

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Biologie Ausgabe 138 Endothelial-Mesenchymale Transition EndMT Endothelzellen TGF-β α-SMA Kollagen MicroRNA
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