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JoVE Journal Developmental Biology
Hele Mount immunofluorescentie en follikel kwantificering van gekweekte muis eierstokken
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Hele Mount immunofluorescentie en follikel kwantificering van gekweekte muis eierstokken

Article DOI: 10.3791/57593-v • 08:15 min • May 2nd, 2018
May 2nd, 2018 •
1, 2, 1,2
1Department of Biomedical Sciences, Cornell University, 2Department of Molecular Biology and Genetics, Cornell University

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Hier presenteren we een protocol om te kwantificeren follikels in gekweekte eierstokken van jonge Muizen zonder seriële segmenteren. Met behulp van hele orgel immunofluorescentie en weefsel wissen, is fysieke segmenteren vervangen door optische segmenteren. Deze methode voor de bereiding van de monsters en visualisatie handhaaft integriteit van orgel en vergemakkelijkt geautomatiseerde kwantificering van specifieke cellen.

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Ontwikkelingsbiologie kwestie 135 reproductieve biologie Mus musculus eierstok cultuur weefsel clearing hele mount beeldvorming immunofluorescentie
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