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JoVE Journal
Developmental Biology

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培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究
 
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培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究

Article DOI: 10.3791/57593-v 08:15 min May 2nd, 2018
May 2nd, 2018

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Summary

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在这里, 我们提出一项协议, 以量化卵泡在培养卵巢小鼠没有连续切片。用全器官免疫荧光和组织清除, 用光学切片代替物理切片。这种样品制备和可视化方法保持了器官的完整性, 促进了特定细胞的自动定量。

Tags

发育生物学 135 期 生殖生物学,毛里求斯大鼠 卵巢培养 组织清除 全芒成像 免疫荧光
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