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Journal / Bioengineering / En Ultrahigh-overførselshastighed mikrofluid…
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Bioengineering

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JoVE Journal Bioengineering
En Ultrahigh-overførselshastighed mikrofluid Platform for encellede Genome Sequencing
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En Ultrahigh-overførselshastighed mikrofluid Platform for encellede Genome Sequencing

Article DOI: 10.3791/57598-v • 10:00 min • May 23rd, 2018
May 23rd, 2018 •
1,2, 1, 1,2, 1,2,3
1Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, 2UC Berkeley-UCSF Graduate Program in Bioengineering, University of California, San Francisco, 3Chan Zuckerberg Biohub

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Encellede sekventering afslører genotypiske heterogenitet i biologiske systemer, men aktuelle teknologier mangler overførselshastighed noedvendigt for deep profilering af Fællesskabets sammensætning og funktion. Her, vi beskriver en mikrofluid arbejdsproces til sekvensering > 50.000 encellede genomer fra forskellige celle populationer.

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Bioteknologi spørgsmålet 135 mikrofluidik microdroplets encellede genomforskning metagenomics næste generation sequencing Molekylær stregkodesystem
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