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Journal / Bioengineering / En av beryllium gjennomstrømming Microfluidic…
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Bioengineering

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JoVE Journal Bioengineering
En av beryllium gjennomstrømming Microfluidic plattform for enkeltceller genomet sekvensering
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En av beryllium gjennomstrømming Microfluidic plattform for enkeltceller genomet sekvensering

Article DOI: 10.3791/57598-v • 10:00 min • May 23rd, 2018
May 23rd, 2018 •
1,2, 1, 1,2, 1,2,3
1Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, 2UC Berkeley-UCSF Graduate Program in Bioengineering, University of California, San Francisco, 3Chan Zuckerberg Biohub

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Encellede sekvensering avslører genotypic heterogenitet i biologiske systemer, men dagens teknologi mangler gjennomstrømningen nødvendig for dyp profilering av samfunnet komposisjon og funksjon. Her beskriver vi en microfluidic arbeidsflyt for sekvensering > 50.000 encellede genomer fra ulike celle populasjoner.

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Bioteknologi problemet 135 Microfluidics microdroplets encellede genomics metagenomics neste generasjons sekvensering molekylær barcoding
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