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Journal / Genetics / Ultralow Input Genome Sequencing bibliotek…
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JoVE Journal Genetics
Ultralow Input Genome Sequencing bibliotek forberedelse fra en enkelt Tardigrade prøvemateriale
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Ultralow Input Genome Sequencing bibliotek forberedelse fra en enkelt Tardigrade prøvemateriale

Article DOI: 10.3791/57615-v • 10:28 min • July 15th, 2018
July 15th, 2018 •
1,2, 1,3, 1,3, 1,3, 1,2,3, 1,2,3
1Institute for Advanced Biosciences, Keio University, 2Systems Biology Program, Graduate School of Media and Governance, Keio University, 3Faculty of Environment and Information Studies, Keio University

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Forurening under den genomisk sekventering af mikroskopiske organismer er fortsat et stort problem. Her viser vi en metode til sequence genomet af et tardigrade fra et enkelt eksemplar med så lidt som 50 pg genomisk DNA uden hele genom forstærkning til at minimere risikoen for kontaminering.

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Genetik sag 137 Ultralow indgang forurening-fri genome sequencing tardigrade Hypsibius dujardini fryse-tø homogenisering
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