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Isolatie van darmen gliale cellen uit de Submucosa en de Lamina Propria van de volwassen muis
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A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
Journal (Biology)
A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
 
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Isolatie van darmen gliale cellen uit de Submucosa en de Lamina Propria van de volwassen muis

Article DOI: 10.3791/57629-v • 07:53 min • August 15th, 2018
August 15th, 2018 •
1,4, 1, 1, 1, 1, 3, 1,2, 1,2,5
1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology, University of Arizona College of Medicine

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Hier beschrijven we de isolatie van darmen-gliale cellen uit de intestinale submucosa-sequentiële EDTA incubations met divalente kationen en vervolgens incubatie in de oplossing van de gegevensterugwinning van de niet-enzymatische cel chelaat. De resulterende celsuspensie op poly-D-lysine en laminin plating resulteert in een sterk verrijkt cultuur van submucosal gliacellen voor functionele analyse.

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Biologie kwestie 138 twaalfvingerige darm hGFAP-Cre tdTomato EDTA chelatie EGCs Ca2 + flux Sox10 algemeen kaderakkoord voor vrede
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