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Cancer Research

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JoVE Journal Cancer Research
Karakterisering van de DNA-herstelsynthese op Transiënten- en Long-lasting Double-streng DNA-einden door immunofluorescentie microscopie
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Karakterisering van de DNA-herstelsynthese op Transiënten- en Long-lasting Double-streng DNA-einden door immunofluorescentie microscopie

Article DOI: 10.3791/57653-v • 08:31 min • June 8th, 2018
June 8th, 2018 •
*1, *1, 2, 1, 1, 1, 1, 1, 1
1Division of Biology, Kansas State University, 2Bristol Medical School, Translational Health Sciences, University of Bristol
* These authors contributed equally

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Reparatie van dubbele streng DNA pauzes is een dynamisch proces, waarbij niet alleen de vorming van reparatie complexen in de pauzes, maar ook hun resolutie na de laesie is gericht. Hier, gebruiken we immunofluorescentie microscopie voor voorbijgaande en langdurige double-stranded pauzes als een instrument om te ontleden dit genoom onderhoud mechanisme.

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Kankeronderzoek kwestie 136 immunofluorescentie microscopie DNA herstellen dubbel-strand einden reparatie kinetiek homologe recombinatie niet-homologe einde toe te treden H2AX
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