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Cancer Research

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JoVE Journal Cancer Research
Modelleren van osteosarcoom met behulp van Li-Fraumeni syndroom geïnduceerde pluripotente stamcellen patiënt-afgeleide cellen
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Modelleren van osteosarcoom met behulp van Li-Fraumeni syndroom geïnduceerde pluripotente stamcellen patiënt-afgeleide cellen

Article DOI: 10.3791/57664-v • 08:52 min • June 13th, 2018
June 13th, 2018 •
1,2, 1, 1,3, 1, 4, 1, 1,2,5,6
1Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, 2Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, 3Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, 4Women's Health Institute, Cleveland Clinic Foundation, 5Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, 6Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston

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Hier presenteren we een protocol voor het genereren van geïnduceerde pluripotente stamcellen (iPSCs) van Li-Fraumeni syndroom (LFS) patiënt afgeleide fibroblasten, differentiatie van iPSCs via mesenchymale stamcellen (MSCs) botcellen te modelleren in vivo tumorigenesis met behulp van LFS patiënt afkomstige botcellen.

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Kankeronderzoek kwestie 136 iPSCs Li-Fraumeni syndroom herprogrammering differentiatie mesenchymale stamcellen botcellen Osteosarcoom xenograft
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