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JoVE Journal Cancer Research
Modelagem de osteossarcoma usando síndrome de Li-Fraumeni paciente-derivado induzido Pluripotent Stem Cells
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Modelagem de osteossarcoma usando síndrome de Li-Fraumeni paciente-derivado induzido Pluripotent Stem Cells

Article DOI: 10.3791/57664-v • 08:52 min • June 13th, 2018
June 13th, 2018 •
1,2, 1, 1,3, 1, 4, 1, 1,2,5,6
1Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, 2Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, 3Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, 4Women's Health Institute, Cleveland Clinic Foundation, 5Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, 6Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston

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Aqui, apresentamos um protocolo para a geração de pluripotentes induzidas (iPSCs) as células-tronco do paciente fibroblastos derivada de síndrome de Li-Fraumeni (LFS), diferenciação de iPSCs através de células-tronco mesenquimais (MSCs) de osteoblastos e modelagem em vivo tumorigênese usando osteoblastos paciente-derivado do LFS.

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Pesquisa sobre o câncer edição 136 iPSCs síndrome de Li-Fraumeni reprogramação diferenciação as células-tronco mesenquimais osteoblastos osteossarcoma xenoenxertos
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