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Journal / Developmental Biology / Isolering av mänskliga livmodercancer…
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Developmental Biology
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JoVE Journal Developmental Biology
Isolering av mänskliga livmodercancer stromaceller för In Vitro Decidualization
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Isolering av mänskliga livmodercancer stromaceller för In Vitro Decidualization

Article DOI: 10.3791/57684-v • 14:55 min • September 1st, 2018
September 1st, 2018 •
*1, *1, 1, 1
1Department of Obstetrics and Gynecology, Center for Reproductive Health Sciences, Washington University School of Medicine
* These authors contributed equally

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Denna studie presenterar en validerad och optimerad förfarande för isolering och kultur av mänskliga livmodercancer stromaceller att föra in vitro- decidualization assay. Ytterligare, denna studie ger en detaljerad metod för att effektivt knockdown en specifik gen som använder siRNAs i mänskliga livmodercancer stromaceller.

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Utvecklingsbiologi fråga 139 utvecklingsbiologi progesteron östrogen cAMP primära kulturer mänskliga livmodercancer stromaceller siRNA transfection och in vitro- decidualization
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