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JoVE Journal Developmental Biology
Onderdrukking van Pro-fibrotische signalering Potentiates Factor-gemedieerde herprogrammering van muis embryonale fibroblasten in geïnduceerde Cardiomyocytes
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Onderdrukking van Pro-fibrotische signalering Potentiates Factor-gemedieerde herprogrammering van muis embryonale fibroblasten in geïnduceerde Cardiomyocytes

Article DOI: 10.3791/57687-v • 09:16 min • June 3rd, 2018
June 3rd, 2018 •
1, 1, 1, 1, 2, 1
1Division of Cardiology, Department of Medicine, University of Colorado Anschutz Medical Campus, 2Department of Population Health Sciences, Medical College of Georgia, Augusta University

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Hier presenteren we een robuuste methode om te herprogrammeren primaire embryonale fibroblasten in functionele cardiomyocytes via overexpressie van GATA4, Hand2, Mef2c, Tbx5, miR-1 en miR-133 (GHMT2m) naast remming van TGF-β signalering. Ons protocol genereert kloppend cardiomyocytes zo spoedig 7 dagen na transductie met korting tot 60% efficiëntie.

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Developmental Biology kwestie 136 Cardiac herprogrammering transcriptiefactoren compound microRNAs pro-fibrotische signalering TGF-β 1 receptor remmer
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