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Developmental Biology

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JoVE Journal Developmental Biology
Undertrykkelse af Pro-fibrotisk signalering Potentiates faktor-medieret omprogrammering af musen embryonale fibroblaster til induceret Cardiomyocytes
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Undertrykkelse af Pro-fibrotisk signalering Potentiates faktor-medieret omprogrammering af musen embryonale fibroblaster til induceret Cardiomyocytes

Article DOI: 10.3791/57687-v • 09:16 min • June 3rd, 2018
June 3rd, 2018 •
1, 1, 1, 1, 2, 1
1Division of Cardiology, Department of Medicine, University of Colorado Anschutz Medical Campus, 2Department of Population Health Sciences, Medical College of Georgia, Augusta University

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Her vil vi præsentere en robust metode til at omprogrammere primære embryonale fibroblaster til funktionel cardiomyocytes gennem overekspression af GATA4, Hand2, Mef2c, Tbx5, miR-1 og miR-133 (GHMT2m) sammen med hæmning af TGF-β signalering. Vores protokol genererer bankende cardiomyocytes så tidligt som 7 dage efter transduktion med op til 60% effektivitet.

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Udviklingsmæssige biologi sag 136 hjerte omprogrammering transkriptionsfaktorer sammensatte MicroRNA pro-fibrotisk signalering TGF-β receptor 1 hæmmer
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