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Flerfärgade lokalisering mikroskopi av enda membranproteiner i organeller av levande däggdjursceller
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Flerfärgade lokalisering mikroskopi av enda membranproteiner i organeller av levande däggdjursceller

Article DOI: 10.3791/57690-v • 11:06 min • June 30th, 2018
June 30th, 2018 •
1, 1, 1, 2, 1,3
1School of Biology, University of Osnabrück, 2Center of Cellular Nanoanalytics, Integrated Bioimaging Facility, University of Osnabrück, 3Department of Biology, WWU Münster

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Här presenterar vi ett protokoll för flerfärgade lokalisering av enda membranproteiner i organeller av levande celler. Bifoga fluorophores genom används egen märkning proteiner. Proteiner, beläget i olika membran fack i samma organell, kan lokaliseras med en precision av ~ 18 nm.

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Biologi fråga 136 enda molekyl spårning enda molekyl lokalisering mitokondrier rodamin färgämnen tvåfärgad super-resolution mikroskopi dynamics av membranproteiner live cell imaging
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