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Journal / Bioengineering / Konjugasyon kaynaklı floresan pegile virüs…
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Bioengineering

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JoVE Journal Bioengineering
Konjugasyon kaynaklı floresan pegile virüs benzeri moleküller tarafından Dibromomaleimide-disülfür Kimya yapma
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Konjugasyon kaynaklı floresan pegile virüs benzeri moleküller tarafından Dibromomaleimide-disülfür Kimya yapma

Article DOI: 10.3791/57712-v • 10:18 min • May 27th, 2018
May 27th, 2018 •
1, 2, 3, 4
1Department of Chemistry & Biochemistry, University of Texas at Dallas, 2Undergraduate Biology, University of Texas at Dallas, 3Undergraduate Healthcare Studies, University of Texas at Dallas, 4Departments of Chemistry & Biochemistry and Biomedical Engineering, University of Texas at Dallas

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Burada, fluorescently Qβ VIP üzerinde disulfides dibromomaleimide ile functionalize için bir yordam mevcut. Biz Qβ ifade ve arıtma, molekülleri dibromomaleimide functionalized sentezi ve dibromomaleimide ve Qβ arasında konjugasyon tepki açıklanmaktadır. Elde edilen Sarı floresan konjuge parçacık bir floresan sonda hücrelere olarak kullanılabilir.

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Biyomühendislik sorunu 135 Nanomalzemeler virüs gibi parçacıklar bakteriyofaj Qβ (Qbeta) functionalizing capsids floresan bioconjugation tepki dibromomaleimide türevleri Polietilen glikol (PEG)
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