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Journal / Bioengineering / Gør konjugation-induceret fluorescerende…
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Bioengineering

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JoVE Journal Bioengineering
Gør konjugation-induceret fluorescerende pegyleret viruslignende partikler af Dibromomaleimide-disulfid kemi
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Gør konjugation-induceret fluorescerende pegyleret viruslignende partikler af Dibromomaleimide-disulfid kemi

Article DOI: 10.3791/57712-v • 10:18 min • May 27th, 2018
May 27th, 2018 •
1, 2, 3, 4
1Department of Chemistry & Biochemistry, University of Texas at Dallas, 2Undergraduate Biology, University of Texas at Dallas, 3Undergraduate Healthcare Studies, University of Texas at Dallas, 4Departments of Chemistry & Biochemistry and Biomedical Engineering, University of Texas at Dallas

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Vi præsenterer her, en procedure for at fluorescently functionalize disulfider på Qβ VLP med dibromomaleimide. Vi beskriver Qβ udtryk og rensning, syntesen af dibromomaleimide-functionalized molekyler og konjugation reaktionen mellem dibromomaleimide og Qβ. Den resulterende gul fluorescerende konjugeret partikel kan bruges som et fluorescens sonde inde i cellerne.

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Bioteknologi spørgsmålet 135 nanomaterialer viruslignende partikler bacteriophage Qβ (Qbeta) functionalizing capsids fluorescens og bioconjugation reaktion dibromomaleimide derivater polyethylenglycol (PEG)
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