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JoVE Journal Bioengineering
Att göra konjugation-inducerad fluorescerande pegylerat virusliknande partiklar av Dibromomaleimide-disulfid kemi
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Att göra konjugation-inducerad fluorescerande pegylerat virusliknande partiklar av Dibromomaleimide-disulfid kemi

Article DOI: 10.3791/57712-v • 10:18 min • May 27th, 2018
May 27th, 2018 •
1, 2, 3, 4
1Department of Chemistry & Biochemistry, University of Texas at Dallas, 2Undergraduate Biology, University of Texas at Dallas, 3Undergraduate Healthcare Studies, University of Texas at Dallas, 4Departments of Chemistry & Biochemistry and Biomedical Engineering, University of Texas at Dallas

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Här presenterar vi ett förfarande för att fluorescently functionalize i disulfides på Qβ VLP med dibromomaleimide. Vi beskriver Qβ uttryck och rening, syntesen av dibromomaleimide-functionalized molekyler och konjugation reaktionen mellan dibromomaleimide och Qβ. Den resulterande gul fluorescerande konjugera partikeln kan användas som en fluorescens sond inuti celler.

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Bioteknik fråga 135 nanomaterial virusliknande partiklar bacteriophage Qβ (Qbeta) funktionaliserar förhoppningsvis fluorescens bioconjugation reaktion dibromomaleimide derivat polyetylenglykol (PEG)
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