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Journal / Biology / 小鼠原发性肾管上皮细胞的分离、鉴定及高通量的细胞外流量分析…
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Biology

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小鼠原发性肾管上皮细胞的分离、鉴定及高通量的细胞外流量分析
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小鼠原发性肾管上皮细胞的分离、鉴定及高通量的细胞外流量分析

Article DOI: 10.3791/57718-v • 09:40 min • June 20th, 2018
June 20th, 2018 •
1,2, 1,2, 2,3,4,5
1Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 2Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 3Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 4Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 5Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine

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该协议提供了一种经济高效的方法, 以分离和表征小鼠原发性肾管细胞, 随后可以进行亚培养, 以评估肾脏生物学功能, 包括线粒体生物能学。

Tags

生物学 问题 136 原细胞分离 原代细胞特征 管状上皮细胞 肾脏 生物能学 线粒体 呼吸 高通量 细胞外流量分析 脂肪酸氧化 慢性肾脏疾病
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