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Journal / Medicine / 低酸素/再酸素化後心筋細胞におけるミトコンドリア膜電位を測定するためのフロー…
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低酸素/再酸素化後心筋細胞におけるミトコンドリア膜電位を測定するためのフロー フローサイトメトリーを用いたアッセイ
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低酸素/再酸素化後心筋細胞におけるミトコンドリア膜電位を測定するためのフロー フローサイトメトリーを用いたアッセイ

Article DOI: 10.3791/57725-v • 07:14 min • July 13th, 2018
July 13th, 2018 •
1, 1, 2, 2
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

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ここでは、JC 1 染料を使用して保護エージェントの有無を低酸素/再酸素化にさらされた後細胞のミトコンドリア膜電位を評価するプロトコルを提案する.

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動作、問題 137 ミトコンドリア膜透過性遷移孔、ミトコンドリアの膜電位、JC 1 アッセイ、再灌流障害、心筋、フローサイトメトリー
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