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Um ensaio de baseados em citometria de fluxo para medir o potencial de membrana mitocondrial em miócitos após hipóxia/reoxigenação
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Um ensaio de baseados em citometria de fluxo para medir o potencial de membrana mitocondrial em miócitos após hipóxia/reoxigenação

Article DOI: 10.3791/57725-v • 07:14 min • July 13th, 2018
July 13th, 2018 •
1, 1, 2, 2
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

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Aqui, apresentamos um protocolo que usa JC-1 tintura para avaliar o potencial de membrana mitocondrial das células após serem expostos a hipóxia/reoxigenação com ou sem um agente protetor.

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Transição de permeabilidade mitocondrial de comportamento edição 137 poros potencial de membrana mitocondrial JC-1 ensaio lesão de reperfusão miócitos citometria de fluxo
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