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Un análisis basado en la citometría de flujo para medir el potencial de membrana mitocondrial en miocitos cardiacos después de hipoxia/Reoxygenation
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Un análisis basado en la citometría de flujo para medir el potencial de membrana mitocondrial en miocitos cardiacos después de hipoxia/Reoxygenation

Article DOI: 10.3791/57725-v • 07:14 min • July 13th, 2018
July 13th, 2018 •
1, 1, 2, 2
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

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Aquí, presentamos un protocolo que utiliza tinte JC-1 para evaluar el potencial de membrana mitocondrial de las células después de ser expuestos a hipoxia/reoxygenation con o sin un agente protector.

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Transición de permeabilidad mitocondrial comportamiento número 137 poro potencial de membrana mitocondrial JC-1 ensayo lesión de la reperfusión miocitos cardiacos citometría de flujo
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