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JoVE Journal Bioengineering
Produktionen af Elastin-lignende Protein Hydrogels til indkapsling og immunfarvning af celler i 3D
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Produktionen af Elastin-lignende Protein Hydrogels til indkapsling og immunfarvning af celler i 3D

Article DOI: 10.3791/57739-v • 11:46 min • May 19th, 2018
May 19th, 2018 •
*1, *2, 1, 2
1Department of Bioengineering, Stanford University, 2Department of Materials Science and Engineering, Stanford University
* These authors contributed equally

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Rekombinante protein-manipuleret hydrogels er fordelagtige for 3D cellekultur, som de giver mulighed for komplet tunability af polymer rygrad og derfor celle mikromiljø. Her, beskriver vi processen med rekombinant elastin-lignende protein oprensning og dens anvendelse i 3D hydrogel celle indkapsling.

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Bioteknologi spørgsmålet 135 Elastin-lignende protein ELP hydrogel 3D celle kultur immunfarvning protein udtryk rekombinante protein
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