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Journal / Bioengineering / Production de protéine élastine Hydrogels pour…
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JoVE Journal Bioengineering
Production de protéine élastine Hydrogels pour Encapsulation et immunomarquage des cellules en 3D
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Production de protéine élastine Hydrogels pour Encapsulation et immunomarquage des cellules en 3D

Article DOI: 10.3791/57739-v • 11:46 min • May 19th, 2018
May 19th, 2018 •
*1, *2, 1, 2
1Department of Bioengineering, Stanford University, 2Department of Materials Science and Engineering, Stanford University
* These authors contributed equally

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Hydrogels d’ingénierie de protéines recombinantes sont avantageuses pour la culture cellulaire 3D car ils permettent accordabilité complète de l’épine dorsale de polymère et par conséquent, le microenvironnement cellulaire. Nous décrivons ici le processus de purification de protéine recombinante élastine et son application dans l’encapsulation de cellules 3D hydrogel.

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Bio-ingénierie numéro 135 Elastin-like protein ELP hydrogel 3D cell culture immunomarquage expression de la protéine protéine recombinante
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