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Journal / Genetics / Biotina-based Pulldown Assay Validate mRNA Mirna…
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Genetics

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JoVE Journal Genetics
Biotina-based Pulldown Assay Validate mRNA Mirna obiettivi del cellulare
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Biotina-based Pulldown Assay Validate mRNA Mirna obiettivi del cellulare

Article DOI: 10.3791/57786-v • 11:00 min • June 12th, 2018
June 12th, 2018 •
1,2,3, 2, 2, 2
1School of Biotechnology, Kalinga Institute of Industrial Technology University, 21. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology, Meharry Medical College, 3Kalinga Institute of Industrial Technology University

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Questo rapporto descrive un metodo veloce e affidabile per la convalida di mRNA bersaglio di Mirna cellulari. Il metodo utilizza biotinilati sintetici basati su Locked Nucleic Acid LNA miRNA imita per catturare destinazione mRNA. Successivamente, biglie magnetiche rivestite con streptavidina sono impiegati per pulldown il bersaglio mRNA per quantificazione da reazione a catena della polimerasi qPCR.

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Genetica problema 136 miRNA mRNA LNA biotinylation PCR 3' UTR
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