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JoVE Journal Genetics
Pulldown biotina-baseado do ensaio para validar mRNA miRNAs alvos de celular
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Pulldown biotina-baseado do ensaio para validar mRNA miRNAs alvos de celular

Article DOI: 10.3791/57786-v • 11:00 min • June 12th, 2018
June 12th, 2018 •
1,2,3, 2, 2, 2
1School of Biotechnology, Kalinga Institute of Industrial Technology University, 21. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology, Meharry Medical College, 3Kalinga Institute of Industrial Technology University

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Este relatório descreve um método rápido e confiável para validar alvos de mRNA de miRNAs celulares. O método usa biotinilado sintético baseado no LNA de ácido nucleico trancado miRNA imita para capturar o alvo do mRNA. Posteriormente, grânulos magnéticos streptavidin-revestidas são empregados para pulldown o alvo mRNA para quantificação por qPCR reação em cadeia da polimerase.

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Genética edição 136 miRNA mRNA LNA biotinylation PCR 3' UTR
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