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Journal / Cancer Research / Immunglobulin-gen Sekvensanalyse i kronisk…
JoVE Journal
Cancer Research

This content is Open Access.

JoVE Journal Cancer Research
Immunglobulin-gen Sekvensanalyse i kronisk lymfatisk leukæmi
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Immunglobulin-gen Sekvensanalyse i kronisk lymfatisk leukæmi: Fra Patient materiale til sekvens fortolkning

Article DOI: 10.3791/57787-v • 09:02 min • November 26th, 2018
November 26th, 2018 •
*1, *2,3, 1, 4, 5, 6, 7, 2,3, 1,2, 8
1Institute of Applied Biosciences, Centre for Research and Technology Hellas, 2Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, 3Department of Molecular Medicine and Surgery, Karolinska Institutet, 4Division of Immunology, Transplantation and Infectious, IRCCS San Raffaele Scientific Institute, 5Department of Immunology, Laboratory for Medical Immunology, Erasmus University Medical Center, 6Hematology Department, Nikea General Hospital, 7Assistance publique - Hôpitaux de Paris (AP-HP), Hopital Pitié-Salpêtrière, Department of Hematology, and UPMC University Paris 06, UMRS 1138, 8Division of Experimental Oncology, IRCCS Istituto Scientifico San Raffaele and Università Vita-Salute San Raffaele
* These authors contributed equally

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Summary
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Heri, præsenterer vi en protokol, der detaljer de tekniske aspekter og væsentlige krav at sikre robuste IG gen Sekvensanalyse hos patienter med kronisk lymfatisk leukæmi (CLL), baseret på den akkumulerede erfaring af initiativet europæisk forskning på CLL (ERIC).

Tags

Kræftforskning sag 141 kronisk lymfatisk leukæmi (CLL) somatiske hypermutation (SHM) immunoglobulin immunoglobulin tunge variabel (IGHV) komplementaritet bestemmelse region 3 (CDR3) internationale ImMunoGeneTics informationssystem (IMGT)
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