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JoVE Journal Neuroscience
Friska hjärnan-hypofys skivor för elektrofysiologiska undersökningar av hypofysen celler i lever fisk
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Friska hjärnan-hypofys skivor för elektrofysiologiska undersökningar av hypofysen celler i lever fisk

Article DOI: 10.3791/57790-v • 07:14 min • August 16th, 2018
August 16th, 2018 •
*1, *1, 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences
* These authors contributed equally

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I artikeln beskrivs en optimerad protokollet för att göra lönsamma hjärnan-hypofys vävnad skivor, använder den lever fisk medaka (Oryzias latipes), följt av elektrofysiologiska inspelningar av hypofysen celler med hjälp av patch-clamp teknik med den perforerad patch konfiguration.

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Neurovetenskap fråga 138 hjärnan-hypofysen elektrofysiologi patch-clamp fisk vävnad-slice endokrina celler
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