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JoVE Journal Bioengineering
Medição da rigidez de substratos de Silicone macio para estudos de Mechanobiology, usando um microscópio de fluorescência Widefield
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Medição da rigidez de substratos de Silicone macio para estudos de Mechanobiology, usando um microscópio de fluorescência Widefield

Article DOI: 10.3791/57797-v • 07:02 min • July 3rd, 2018
July 3rd, 2018 •
*1, *1, *2, *1, 1, 1
1Department of Mechanical & Aerospace Engineering, Old Dominion University, 2Department of Biological Sciences, Old Dominion University
* These authors contributed equally

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Substratos com rigidez na faixa de superfície são úteis para estudar a resposta das células à rigidez de microambiente fisiologicamente relevante. Usando apenas um microscópio de fluorescência widefield, o módulo de Young de gel de silicone suave pode ser determinado usando um recuo com uma esfera apropriada.

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Bioengenharia edição 137 recuo polidimetilsiloxano microscopia de força de tração fluorescência mechanobiology biomecânica
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