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Journal / Biochemistry / 高效纯化和基于 LC ms/ms 的十-十一 Translocation-2…
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Biochemistry

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JoVE Journal Biochemistry
高效纯化和基于 LC ms/ms 的十-十一 Translocation-2 5-甲基胞嘧啶酶的检测开发
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高效纯化和基于 LC ms/ms 的十-十一 Translocation-2 5-甲基胞嘧啶酶的检测开发

Article DOI: 10.3791/57798-v • 10:34 min • October 15th, 2018
October 15th, 2018 •
*1, *1, *1, 1, 1
1Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City
* These authors contributed equally

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在这里, 我们提出了一个有效的单步纯化活性无标签人的协议十-十一 translocation-2 (TET2) 5-甲基胞嘧啶酶使用离子交换色谱法及其测定液相色谱-串联质量基于质谱 (LC-ms) 的方法。

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生物化学 问题 140 十-十一易位 去甲基酶 白血病 酶 LC-ms 表观遗传学 转录调节
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