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JoVE Journal Biochemistry
Purification efficace et LC-MS/MS-based test développement pour dix-onze Translocation-2 5-méthylcytosine Dioxygenase
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Purification efficace et LC-MS/MS-based test développement pour dix-onze Translocation-2 5-méthylcytosine Dioxygenase

Article DOI: 10.3791/57798-v • 10:34 min • October 15th, 2018
October 15th, 2018 •
*1, *1, *1, 1, 1
1Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City
* These authors contributed equally

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Nous présentons ici un protocole pour une purification efficace seule étape de l’humain sans balise active dix-onze translocation-2 (TET2) 5-méthylcytosine dioxygénase utilisant la chromatographie échangeuse d’ions et son dosage à l’aide d’une masse en tandem par chromatographie liquide spectrométrie (LC-MS/MS)-approche de base.

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Biochimie numéro 140 dix-onze Translocation déméthylase leucémie dioxygénase LC-MS/MS épigénétique régulation de la Transcription
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