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JoVE Journal Biochemistry
酵母 2-混合筛在批次中比较蛋白质相互作用
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Journal (Biochemistry)
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Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
Journal (Biochemistry)
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
 
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酵母 2-混合筛在批次中比较蛋白质相互作用

Article DOI: 10.3791/57801-v • 14:23 min • June 6th, 2018
June 6th, 2018 •
1, 1, 1
1Molecular Physiology and Biophysics, University of Iowa

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酵母 2-混合筛的分批处理可以直接比较多饵蛋白与高度复杂的猎物融合蛋白的相互作用。在这里, 我们描述了精制方法, 新试剂, 以及如何实现这些屏幕的使用。

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生物化学 问题 136 蛋白质相互作用 下一代测序 DNA 序列分析 酵母 2-杂交
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