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Journal / Biochemistry / Små kolorimetriske Assays af intracellulære…
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Biochemistry

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JoVE Journal Biochemistry
Små kolorimetriske Assays af intracellulære laktat og pyruvat i at ødelægge Caenorhabditis elegans
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Små kolorimetriske Assays af intracellulære laktat og pyruvat i at ødelægge Caenorhabditis elegans

Article DOI: 10.3791/57807-v • 09:35 min • October 15th, 2018
October 15th, 2018 •
1,2, 3, 3
1Department of Health Science, School of Sports & Health Science, Daito Bunka University, 2Department of Molecular Life Science, School of Medicine, Tokai University, 3Department of Health Management, School of Health Study, Tokai University

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Vi beskriver en modificeret små udvinding og kolorimetriske assays af laktat og pyruvat i fyrretræsnematoden C. elegans. Når udnytte kommerciel assay kits, er den tekniske udvikling af deres følsomhed og nøjagtighed vigtige. Protein nedbør i udvinding er det mest afgørende skridt for kvantitativ bestemmelse af intracellulære metabolitter.

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Biokemi spørgsmål 140 Caenorhabditis elegans energimetabolisme laktat pyruvat fælles fældningsmiddel kolorimetrisk assay
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