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Journal / Genetics / 一种新的饱和诱变方法: 利用 Phosphorothioates 对 RNA…
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JoVE Journal Genetics
一种新的饱和诱变方法
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一种新的饱和诱变方法: 利用 Phosphorothioates 对 RNA 调控蛋白结合位点的单步表征

Article DOI: 10.3791/57816-v • 11:49 min • August 21st, 2018
August 21st, 2018 •
1
1Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder

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结合特定 RNA 序列的蛋白质在基因表达中扮演重要角色。对这些结合部位的详细描述对于我们理解基因调控至关重要。本文介绍了 RNA 蛋白质结合部位饱和诱变的单步法方法。这种方法与 RNA 中的所有蛋白结合部位有关。

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遗传学 问题 138 RNA 结合蛋白 DNA 结合蛋白 phosphorothioates 饱和诱变 楔凝胶电泳 显影 掺杂
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