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JoVE Journal
Genetics

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一种组合单细胞法表征人茎和祖种群的分子和免疫表型异质性
 
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一种组合单细胞法表征人茎和祖种群的分子和免疫表型异质性

Article DOI: 10.3791/57831-v 09:34 min October 25th, 2018
October 25th, 2018

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Summary

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大体积基因表达测量在异构细胞种群中云个体细胞差异。在这里, 我们描述了一个关于如何通过花期活化细胞分类 (immunophenotypically) 的单细胞基因表达分析和指数排序的协议, 以描绘异质性和分子不同细胞种群的特征。

Tags

遗传学 问题 140 异质性 单细胞 rt-pcr 流式细胞 指数排序 免疫表型表征 基因表达分析
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