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Journal / Biochemistry / Proteinekspression og -oprensning af pattedyr…
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Biochemistry

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JoVE Journal Biochemistry
Proteinekspression og -oprensning af pattedyr Bestrophin Ion kanaler
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Proteinekspression og -oprensning af pattedyr Bestrophin Ion kanaler

Article DOI: 10.3791/57832-v • 08:12 min • August 2nd, 2018
August 2nd, 2018 •
1, 1, 1, 1, 1
1Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry

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Rensning af Ionkanaler er ofte udfordrende, men når opnået, kan det potentielt give in vitro- undersøgelser af funktioner og strukturer af kanaler. Her beskriver vi trinvis procedurerne for den proteinekspression og -oprensning af pattedyr bestrophin proteiner, en familie af Ca2 +-aktiveret Cl- kanaler.

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Biokemi spørgsmålet 138 pattedyr membran protein ionkanaler retinal degenerative sygdomme protein udtryk protein oprensning Bestrophin-1 BEST1 Ca2 +-aktiveret Cl- kanal (CaCC)
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