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Journal / Biochemistry / Uttryck och rening av däggdjur Bestrophin…
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Biochemistry

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JoVE Journal Biochemistry
Uttryck och rening av däggdjur Bestrophin jonkanaler
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Uttryck och rening av däggdjur Bestrophin jonkanaler

Article DOI: 10.3791/57832-v • 08:12 min • August 2nd, 2018
August 2nd, 2018 •
1, 1, 1, 1, 1
1Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry

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Rening av jonkanaler är ofta utmanande, men när uppnått, det potentiellt kan tillåta in vitro- undersökningar av funktioner och strukturer av kanalerna. Här, vi beskriver stegvis förfarandena för uttryck och rening av däggdjur bestrophin proteiner, en familj av Ca2 +-aktiverade Cl– kanaler.

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Biokemi fråga 138 däggdjur membranprotein jonkanaler retinala degenerativa sjukdomar proteinuttryck protein rening Bestrophin-1 BEST1 Ca2 +-aktiverade Cl– kanal (CaCC)
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