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Co-immunoprecipitation analysen bruke endogene kjernefysiske proteiner fra celler kultivert Hypoxic vilkår
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Co-immunoprecipitation analysen bruke endogene kjernefysiske proteiner fra celler kultivert Hypoxic vilkår

Article DOI: 10.3791/57836-v • 09:17 min • August 2nd, 2018
August 2nd, 2018 •
1,2, 1, 3, 4, 5, 3, 1,2,3, 1,2
1Lee Kong Chian School of Medicine, Nanyang Technological University, 2Singapore Eye Research Institute (SERI), Singapore General Hospital, 3The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, 4Cancer and Stem Cell Biology Program, Duke-NUS Medical School, 5Department of Cell and Molecular Biology, Karolinska Institutet

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Her beskriver vi en co-immunoprecipitation-protokoll for å studere protein-protein interaksjoner mellom endogene kjernefysiske proteiner under hypoxic forhold. Denne metoden er egnet for demonstrasjon av samspillet mellom transkripsjonsfaktorer og transcriptional co regulatorer på hypoksi.

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Biologi problemet 138 molekylærbiologi co-immunoprecipitation protein-protein interaksjoner (PPIs) hypoksi hypoksi-induserbart faktorer (HIFs) endogen proteiner kjernefysiske ekstrakter
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